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Updated: Aug 25, 2026
RNAscope for In situ Detection of Transcriptionally Active Human Papillomavirus in Head and Neck Squamous Cell Carcinoma
Published on: March 11, 2014
[Detection of high risk human papillomavirus DNA in oral squamous cell carcinoma]
1Departmet Of Oral & Maxillofacial Surgery, Ninth People's Hospital, Shanghai Second Medical University, Shanghai 200011, China.
Objective:
To ascertain the prevalence of human papillomavirus(HPV) DNA in oral squamous cell carcinomas(OSCCs) and normal oral mucosa and to correlate the virologic data with other factors obtained from patients' records.
Methods:
113 paraffin embedded tissue blocks including 73 OSCCs and 40 normal oral mucosa were studied by polymerase chain reaction (PCR) for HPV type 16 and 18 DNA. Medical records of patients were reviewed to obtained the following factors: age, sex, tobacco and alcohol usage, site of tumor, histologic grade and clinical stage.
Results:
74%(54/73) of OSCCs and 55%(22/40) of normal oral mucosa were HPV 16/18 DNA positive. The presence of HPV DNA showed significant difference between OSCCs and normal oral mucosa (P=0.040). Statistical analysis indicated that age (60
Conclusion:
High risk HPV genotypes have a significant association with oral squamous cell carcinoma. It is a co-factor in oral carcinogenesis, particularly in male patients and patients under the sixth decade. HPV infection in the oral mucosa is common. HPV is not the single etiological factor responsible for human oral neoplasia.
