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Published on: March 29, 2018
[Expression of matrixmetalloproteinase-8 on the bell-stage in human and rat tooth development]
Yu-qing Hao1, Zhong-ying Niu, Gou-quan Wang
1Key Lab. of Oral Biomedical Engineering Ministy of Education, Sichuan University, Chengdu 610041, China.
Objective:
To study the expression of MMP-8 in human and rat tooth development.
Methods:
Immunohistochemistry was used to detect the localization of MMP-8 protein while in situ hybridization was used to examine the expression of MMP-8 mRNA.
Results:
The expression of MMP-8 protein was localized in odontoblast and dentin matrix at the later bell stage in human tooth germ. The dentin was denser close to the pulp cavity. The expression of MMP-8 mRNA was found in very few polarized odontoblast at the early bell stage and all polarized odontoblast at the later bell stage in rat tooth germ.
Conclusion:
The results suggested that MMP-8 involved in dentin matrix rebuilding in the process of dentin formation in human and rat dental development.
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