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[Analysis of human growth hormone-binding protein in plasma by high pressure liquid chromatography]
A Tar1, J F Hocquette, J C Souberbielle
1INSERM U.30, Hôpital des Enfants-Malades, Paris.
Orvosi Hetilap
|March 1, 1992
Abstract:
A technique using high pressure liquid chromatography gel filtration was used to evaluate GH-binding proteins (GH-BPs) in human plasma; eluate was monitored for radioactivity in a gamma-detection system connected to a computer. Plasma (200 microliters) was incubated with 125I-human (h) GH (200,000 cpm) at 4 degrees C for 20 hours. Our GH binding assay offers important gains in terms of rapidity and resolution; it has permitted a clear separation and characterization of the two GH-binding components present in human plasma.