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Pasteurella multocida contains multiple immunogenic haemin- and haemoglobin-binding proteins
Montserrat Bosch1, M Elena Garrido, Ana M Pérez de Rozas
1Department of Genetics and Microbiology, Universitat Autònoma de Barcelona, Bellaterra, 08193 Barcelona, Spain.
Abstract:
Iron-dependent outer membrane proteins (IROMPs) play an important role in bacterial pathogenesis and present several attributes of potential vaccine candidates. TBLASTN analysis of the Pasteurella multocida Pm70 genome using the same molecules of other bacterial pathogens as a query identified eight putative haemin and haemoglobin receptors for this organism. Quantitative binding assays have demonstrated that the proteins PM0040, PM0236, PM0741, PM1081, PM1428, PM0592 and HgbA bind both haemin and haemoglobin, whereas PM0576 and PM1282 ORFs only bind either haemoglobin or haemin, respectively. Furthermore, Western blot analysis showed that P. multocida-infected mice generate specific antibodies against PM0040, PM0236, PM0741, PM1081, PM1428, PM0592 and HgbA proteins. Nevertheless, inoculation of mice with any single one of these receptors alone did not protect against P. multocida infection.
Insights
Iron-dependent outer membrane proteins (IROMPs) are key in Pasteurella multocida pathogenesis. While antibodies target these receptors, single IROMPs do not confer protection against infection.
Area of Science:
- Microbiology
- Immunology
- Bacterial Pathogenesis
Background:
- Iron-dependent outer membrane proteins (IROMPs) are crucial for bacterial survival and virulence.
- These proteins are potential targets for vaccine development against bacterial infections.
Purpose of the Study:
- To identify and characterize iron-dependent outer membrane proteins in Pasteurella multocida Pm70.
- To evaluate the immunogenicity and protective efficacy of these receptors as vaccine candidates.
Main Methods:
- TBLASTN analysis of the P. multocida Pm70 genome to identify putative IROMPs.
- Quantitative binding assays to assess haemin and haemoglobin binding.
- Western blot analysis to detect host antibody responses in infected mice.
Main Results:
- Eight putative haemin and/or haemoglobin receptors were identified in P. multocida.
- Seven proteins (PM0040, PM0236, PM0741, PM1081, PM1428, PM0592, HgbA) bound both haemin and haemoglobin.
- Infection elicited specific antibodies against these receptors, but single receptor immunization did not protect mice.
Conclusions:
- P. multocida utilizes multiple IROMPs for iron acquisition.
- While immunogenic, individual IROMPs are insufficient for protective immunity against P. multocida infection.
- Further research into combination vaccines targeting multiple IROMPs may be warranted.
