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Streptococcus mutans fructosyltransferase interactions with glucans.

Ramona Rozen1, Doron Steinberg, Gilad Bachrach

  • 1Institute of Dental Sciences, Faculty of Dentistry, Hebrew University-Hadassah, P.O. Box 12272, Jerusalem 91120, Israel.

FEMS Microbiology Letters
|March 17, 2004
PubMed
Summary
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Streptococcus mutans secretes fructosyltransferase (FTF) when grown on sucrose, which binds to glucans. This suggests FTF and glucans are key components of oral biofilm matrix.

Area of Science:

  • Microbiology
  • Oral Health
  • Biochemistry

Background:

  • Streptococcus mutans synthesizes glucans and fructans from sucrose using specific enzymes.
  • Fructosyltransferase (FTF) is an enzyme produced by S. mutans.
  • Understanding FTF secretion and function is crucial for studying oral biofilms.

Purpose of the Study:

  • To investigate the secretion of fructosyltransferase (FTF) by Streptococcus mutans.
  • To determine the interaction of FTF with bacterial cells and extracellular matrix components.

Main Methods:

  • Antibodies against recombinant FTF were used to study its secretion.
  • S. mutans cultures grown with different sugars were analyzed for cell-free and cell-surface FTF.
  • Adsorption assays were performed using recombinant FTF and various bacterial strains/conditions.

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Main Results:

  • Low levels of cell-free FTF were detected in sucrose-grown cultures.
  • Increased cell-surface FTF was observed on S. mutans grown with sucrose.
  • FTF adsorbed to sucrose-grown S. mutans but not to those grown on glucose/fructose or a gtf mutant.
  • Recombinant FTF showed high affinity for glucans.

Conclusions:

  • Sucrose availability influences FTF localization in S. mutans cultures.
  • FTF binding to glucans suggests a role in the oral biofilm matrix.
  • Fructans and glucans are integral components of the polysaccharide matrix in oral biofilms.