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Updated: Aug 25, 2026

A Multilabel Single Molecule Localization Microscopy Protocol for Investigation of Chromatin in the Dense Nuclear Environment
Published on: June 5, 2026
To the 30-nm chromatin fiber and beyond
Nicholas L Adkins1, Meagan Watts, Philippe T Georgel
1Department of Biological Sciences, College of Science, Marshall University, 1 John Marshall Drive, Huntington, WV 25701, USA.
Abstract:
Chromatin fibers are intrinsically dynamic macromolecular complexes whose biological functions are intimately linked with their structure and interactions with chromatin-associated proteins (CAPs). Three-dimensional architectural transitions between or within the two co-existing chromatin types referred to as euchromatin and heterochromatin have been associated with activation or repression of nuclear functions. The presence of specific subsets of chromosomal proteins co-existing with the different chromatin conformations suggests a functional significance for their co-localization. The major points of emphasis of this review will assess the structure, function and recently documented exchanges amongst various members of the CAP family.
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