Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

ProteinChip array-based amyloid beta assays.

Lisa E Bradbury1, James F LeBlanc, Diane B McCarthy

  • 1Ciphergen Biosystems, Inc, Fremont, CA, USA.

Methods in Molecular Biology (Clifton, N.J.)
|March 17, 2004
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

N-acetylglucosaminylation of serine-aspartate repeat proteins promotes Staphylococcus aureus bloodstream infection.

The Journal of biological chemistry·2013
Same author

Apolipoprotein A1 and C-terminal fragment of α-1 antichymotrypsin are candidate plasma biomarkers associated with acute renal allograft rejection.

Transplantation·2011
Same author

Proteomic analysis reveals innate immune activity in intestinal transplant dysfunction.

Transplantation·2011
Same author

Serum protein expression profiling for cancer detection: validation of a SELDI-based approach for prostate cancer.

Disease markers·2004
Same author

3D electron microscopy reveals the variable deposition and protein dynamics of the peripheral pyruvate dehydrogenase component about the core.

Proceedings of the National Academy of Sciences of the United States of America·2003
Same author

DNA affinity capture and protein profiling by SELDI-TOF mass spectrometry: effect of DNA methylation.

Nucleic acids research·2002
Same journal

Mapping the 3D Chromosome Organization of a Biosynthetic Gene Cluster by Capture Hi-C (CHi-C).

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Mapping the 3D Chromosome Organization of Streptomyces by Hi-C.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

CUT&Tag Epigenomic Profiling of Biosynthetic Gene Clusters in Arabidopsis thaliana.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Rhizobium rhizogenes-Mediated Hairy Root Transformation Protocol for Lotus japonicus and Other Legumes.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Characterization of Bioactive Saponins from Sea Cucumbers.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Methods for Functional Validation of Terpenoid Metabolic Clusters in Nicotiana benthamiana and Aspergillus oryzae.

Methods in molecular biology (Clifton, N.J.)·2026
See all related articles

Detecting amyloid-beta (A beta) fragments in Alzheimer's disease is challenging. A novel ProteinChip Array assay coupled with SELDI-TOF MS enables sensitive detection of multiple A beta fragments in patient samples.

Area of Science:

  • Biochemistry
  • Neuroscience
  • Analytical Chemistry

Background:

  • Amyloid-beta (A beta) fragments are key pathological hallmarks in Alzheimer's disease (AD).
  • Accumulation of A beta peptides in the brain and cerebrospinal fluid (CSF) is characteristic of AD.
  • Standard detection methods struggle with the small size and multiple isoforms of A beta peptides.

Purpose of the Study:

  • To develop a sensitive assay for detecting multiple amyloid-beta fragments.
  • To overcome limitations of current methods for A beta detection in Alzheimer's disease.

Main Methods:

  • Utilized a ProteinChip Array-Based assay with immobilized antibodies specific to amyloid epitopes.
  • Applied crude biological samples (lysates, serum, CSF) to antibody-coated arrays.

Related Experiment Videos

  • Employed surface-enhanced laser desorption/ionization time-of-flight mass spectrometry (SELDI-TOF MS) for fragment detection and mass-based resolution.

    • Main Results:

    • The ProteinChip Array assay successfully retained A beta peptides from complex biological samples.
    • SELDI-TOF MS effectively resolved and detected multiple A beta peptide fragments based on their distinct masses.
    • This method demonstrated enhanced capability for detecting various A beta fragments compared to standard techniques.

    Conclusions:

    • A ProteinChip Array assay combined with SELDI-TOF MS offers a powerful tool for detecting multiple amyloid-beta fragments.
    • This approach facilitates improved diagnosis and research for Alzheimer's disease by enabling sensitive A beta detection.