[Strategic approach to glycohemoglobin determination]

Area of Science:

• Clinical Chemistry
• Endocrinology
• Diabetes Mellitus Research

Background:

• Diabetes Mellitus (DM) requires continuous monitoring of metabolic control.
• Glycated hemoglobin (GH) is a key biomarker for assessing long-term glycemic status.
• Accurate quantification of GH is essential for effective DM management.

Purpose of the Study:

• To design an approach for the clinical application of glycated hemoglobin (GH) in patients with diabetes mellitus (DM).
• To evaluate the relationship between GH levels and various clinical parameters in DM patients.
• To compare different methods for GH quantification.

Main Methods:

• Examined 139 patients with Type 1 and Type 2 DM.
• Quantified GH using four distinct methods: immunochemistry, colorimetry, oxalic and phosphoric acids, and isoelectrofocusing.
• Employed correlation and regression analyses to assess relationships between GH and other parameters.

Main Results:

• GH levels demonstrated a non-linear association with daily glucosuria, daily proteinuria, total serum cholesterol, body weight index, and disease duration.
• No significant correlation was found between GH levels and accidental pre- and postprandial glycemia.
• Comparative analysis highlighted the reliability of GH as a metabolic indicator.

Conclusions:

• Glycated hemoglobin (GH) remains a dependable criterion for assessing profound metabolic conditions in diabetes mellitus (DM).
• The quantification of GH requires the use of validated, argumentative methods.
• Clinical decisions should consider the comprehensive factors influencing GH levels.