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Related Experiment Videos

Isolation, characterisation and antibiogram of Enterococci from clinical samples.

S Parvathi1, B Appalaraju

  • 1Department of Microbiology, P.S.G. Institute of Medical Sciences and Research, Coimbatore, Tamilnadu.

Indian Journal of Pathology & Microbiology
|March 18, 2004
PubMed
Summary

This study investigated enterococci prevalence in clinical samples, finding a 3.38% isolation rate. Enterococcus faecalis was the most common species, with high vancomycin sensitivity observed in isolates.

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Area of Science:

  • Microbiology
  • Clinical Diagnostics
  • Infectious Diseases

Background:

  • Enterococci are significant opportunistic pathogens.
  • Understanding their prevalence in clinical settings is crucial for infection control.
  • Enterococcus faecalis is a common species associated with healthcare-associated infections.

Purpose of the Study:

  • To determine the prevalence of enterococci in various clinical specimens.
  • To identify the most common species of enterococci isolated.
  • To assess the antimicrobial susceptibility of enterococci isolates, particularly to vancomycin.

Main Methods:

  • Processing of 2955 clinical specimens including urine, pus, wound swabs, and others over one year.
  • Isolation and speciation of enterococci using conventional microbiological methods.

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  • Antimicrobial susceptibility testing via the standard disc diffusion method.

    • Main Results:

    • One hundred (3.38%) Enterococcus isolates were obtained from 2955 specimens.
    • Highest incidence observed in urine (43%) and pus (40%) specimens.
    • Enterococcus faecalis comprised 88% of all Enterococcus isolates, with 95% vancomycin sensitivity.

    Conclusions:

    • Enterococci are prevalent in clinical specimens, particularly from urine and pus.
    • Enterococcus faecalis is the dominant species, indicating its clinical significance.
    • High vancomycin sensitivity suggests its continued utility in treating enterococcal infections, though monitoring is essential.