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Nitrilotriacetic acid-modified magnetic nanoparticles as a general agent to bind histidine-tagged proteins
Chenjie Xu1, Keming Xu, Hongwei Gu
1Department of Chemistry, Department of Physics, and Bioengineering Program, The Hong Kong University of Science and Technology, Clear Water Bay, Hong Kong, China. chbingxu@ust.hk
Abstract:
Using Nalpha,Nalpha-bis(carboxymethyl)lysine to react with FePt magnetic nanoparticles, we synthesized the FePt-NTA conjugate, which immobilizes Ni2+ ions and selectively binds to histidine-tagged proteins at concentration as low as 0.5 pM. This simple system serves as a useful alternative to existing protocols for protein separation and also acts as a versatile agent for transporting and anchoring proteins.
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