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Base pairing properties of D- and L-cyclohexene nucleic acids (CeNA)
Ping Gu1, Guy Schepers, Jef Rozenski
1Laboratory for Medicinal Chemistry, Rega Institute for Medical Research, B-3000 Leuven, Belgium.
Abstract:
Cyclohexene nucleic acids (CeNA) with a D-like configuration form very stable self-complementary duplexes and stable duplexes with RNA. An increased duplex stability with Delta T(m)/mod of +1.2 degrees C is observed. The duplex with DNA is less stable. Excellent mismatch discrimination has been observed as well for the duplex with DNA as for the duplex with RNA. The results obtained with mixed CeNA sequences warrant antisense studies with CeNA. The CeNAs of opposite chirality constitute a self-pairing system on their own, resembling L-RNA sequences.
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