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Published on: June 1, 2012
A study on a chitosan-gelatin-hyaluronic acid scaffold as artificial skin in vitro and its tissue engineering
Haifeng Liu1, Jinshu Mao, Kangde Yao
1Research Institute of Polymer Materials, Tianjin University, Tianjin 300072, Peoples Republic of China.
Abstract:
Chitosan-gelatin-hyaluronic acid scaffolds for tissue regeneration were fabricated by freezing and lyophilizing methods. The scaffolds showed a higher water uptake and retention abilities than chitosan-gelatin scaffolds did. Fibroblasts cultured in chitosan-gelatin-hyaluronic acid scaffolds grew and proliferated well, and they exhibited a strong viability. Keratinocytes were co-cultured with fibroblasts in chitosan-gelatin-hyaluronic acid scaffolds to construct an artificial bilayer skin in vitro. The artificial skin obtained was flexible and had good mechanical properties. The data from this study suggested that chitosan-gelatin-hyaluronic acid scaffolds are suitable for preparing a bilayer skin substitute.
