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In situ Subcellular Fractionation of Adherent and Non-adherent Mammalian Cells
Published on: July 23, 2010
Cell cycle-related variation in subcellular localization of eIF3e/INT6 in human fibroblasts
1University of Oxford, Sir William Dunn School of Pathology, Oxford, UK.
Cell Proliferation
|March 20, 2004
Summary
The INT6 protein, a component of eukaryotic translation initiation factor eIF3, is found in the nucleus. Its reduced nuclear presence in human cells may signal malignancy.
Area of Science:
- Cell Biology
- Molecular Biology
- Cancer Research
Background:
- The Int-6 gene is a target for mouse mammary tumour virus (MMTV) integration in murine tumors.
- INT6 protein is identified as eIF3e, a subunit of eukaryotic translation initiation factor eIF3.
- INT6 interacts with the COP9 signalosome (CSN) and proteasome complexes.
Purpose of the Study:
- To investigate the subcellular localization of eIF3e/INT6.
- To explore the potential role of eIF3e/INT6 redistribution in human cell malignancy.
Main Methods:
- Immunofluorescence microscopy was employed to visualize eIF3e/INT6 localization.
- Comparison of nuclear INT6 staining in primary human fibroblasts versus transformed cells.
Main Results:
- eIF3e/INT6 exhibits partial nuclear localization, distinct from other cytoplasmic eIF3 components.
- Primary human fibroblasts showed reduced nuclear INT6 staining, particularly in early S phase, compared to transformed cells.
- The protein sequence suggests the presence of both nuclear export and localization signals.
Conclusions:
- The observed variation in eIF3e/INT6 nuclear localization suggests regulated shuttling between cellular compartments.
- Dysregulation of eIF3e/INT6 redistribution may be a key characteristic of human cell malignancy.
