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MS2-Affinity Purification Coupled with RNA Sequencing in Gram-Positive Bacteria
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Optimization of a two-plasmid system for the identification of promoters recognized by RNA polymerase containing

Dagmar Homerova1, Markus Bischoff, Alexis Dumolin

  • 1Institute of Molecular Biology, Center of Excellence for Molecular Medicine, Slovak Academy of Sciences, Dubravska cesta 21, 845 51 Bratislava, Slovak Republic.

FEMS Microbiology Letters
|March 23, 2004
PubMed

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Bacterial RNA Polymerase

Unlike eukaryotes, bacteria use a single RNA Polymerase (RNAP) to transcribe all genes. The different subunits of bacterial RNAPhave distinct functions. The multisubunit structure of the bacterial RNAP helps the enzyme to maintain catalytic function, facilitate assembly, interact with DNA and RNA, and self-regulate its activity.
In most genes, the transcription site is a single base present upstream of the coding sequence. Though RNAP is a catalytically efficient enzyme, it does not recognize...