Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Canine prostate stimulates osteoblast function using the endothelin receptors.

Bruce E LeRoy1, Rani S Sellers, Thomas J Rosol

  • 1Department of Pathology, University of Georgia, Athens, Georgia, USA.

The Prostate
|March 26, 2004
PubMed
Summary

Prostate cancer in dogs and humans can cause new bone formation at metastatic sites. Canine prostate tissue stimulates osteoblast activity through an endothelin-dependent pathway, not involving PTHrP or prostaglandins.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Dual-adjuvant mucosal vaccine leveraging mast cell and TLR9 agonists for protection against poxvirus infection.

RSC pharmaceutics·2026
Same author

Phase variable colony morphotypes of <i>Clostridioides difficile</i> elicit distinct host responses during acute infection.

Infection and immunity·2026
Same author

Preclinical Evaluation of PSMA-Targeted Ultrasound Contrast Agents in an Orthotopic Model of Prostate Cancer in Rabbits.

bioRxiv : the preprint server for biology·2026
Same author

Toxicologic Pathology Forum Opinion*: In Defense of Scientific Freedom.

Toxicologic pathology·2026
Same author

A review of spontaneous bone metastases in domestic and laboratory animals.

Journal of bone oncology·2026
Same author

T cell receptor signaling induces expression of lysine demethylase KDM6B to maintain Treg homeostasis.

The Journal of clinical investigation·2026

Area of Science:

  • Oncology
  • Endocrinology
  • Cell Biology

Background:

  • Bone metastases are common in advanced prostate cancer in humans and dogs, characterized by new bone formation (osteoblastic metastases).
  • Unlike many cancers that cause bone destruction, prostate cancer promotes bone formation, a mechanism not fully understood.
  • Prostate cancer's induction of bone formation is hypothesized to involve paracrine actions of growth factors from prostate epithelial cells.

Purpose of the Study:

  • To investigate the mechanisms underlying prostate-induced new bone formation.
  • To develop an in vitro system to study prostate-stimulated osteoblast function, complementing existing in vivo models.

Main Methods:

  • Utilized an in vitro system with cultured rat calvaria treated with proteins from normal canine prostate tissue.

Related Experiment Videos

  • Measured alkaline phosphatase (ALP) activity as an indicator of osteoblast stimulation.
  • Investigated the role of endothelin, parathyroid hormone-related protein (PTHrP), and prostaglandins using specific antagonists and antibodies.

    • Main Results:

    • Canine prostate proteins significantly stimulated ALP activity in a dose-dependent manner (4-6 fold) within 24 hours.
    • Stimulation was specific to prostate tissue; homogenates from salivary gland, kidney, bladder, and muscle did not induce ALP activity.
    • Endothelin antagonists abrogated the prostate-induced ALP stimulation, while anti-PTHrP antibody and indomethacin did not.

    Conclusions:

    • Osteoblast activation by canine prostate is mediated by an endothelin-dependent mechanism.
    • PTHrP or prostaglandin synthase-mediated pathways are not involved in this process.
    • This study establishes a reliable in vitro assay for studying prostate-mediated osteoblast activation.