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Calibrated real-time PCR for evaluation of parvovirus b19 viral load

Giorgio Gallinella1, Francesca Bonvicini, Claudia Filippone

  • 1Department of Clinical and Experimental Medicine, Division of Microbiology, University of Bologna, Bologna, Italy. ggalline@med.unibo.it

Clinical Chemistry
|March 27, 2004
PubMed
Abstract

No abstract available in PubMed .

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Real Time RT-PCR

Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
The real-time quantification of the number of amplified products is...