Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Experimentally based topology models for E. coli inner membrane proteins.

Mikaela Rapp1, David Drew, Daniel O Daley

  • 1Department of Medical Biochemistry and Biophysics, Karolinska Institutet, SE-171 77 Stockholm, Sweden.

Protein Science : a Publication of the Protein Society
|March 27, 2004
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

pCODE tRNA Expression Plasmids Compensate for Rare Codons in Recombinant Protein Production.

ACS synthetic biology·2026
Same author

Calmodulin assists during co-translational folding of the K<sub>V</sub>7.2 channel calcium responsive domain.

Protein science : a publication of the Protein Society·2026
Same author

A two-step mechanism for sugar translocation.

Nature structural & molecular biology·2026
Same author

Cotranslational Folding and "Constrained Monomers" in the Maturation of HIV-1 Protease.

Journal of molecular biology·2026
Same author

Quasi-continuous cotranslational compaction and folding of a multidomain protein.

bioRxiv : the preprint server for biology·2026
Same author

SARS-CoV-2 membrane protein biogenesis.

bioRxiv : the preprint server for biology·2026

Determining the C-terminus location improves membrane protein topology predictions. This method is effective for large-scale studies, yielding models for 31 E. coli inner membrane proteins.

Area of Science:

  • Biochemistry
  • Structural Biology
  • Genomics

Background:

  • Accurate prediction of membrane protein topology is crucial for understanding protein function.
  • Existing topology prediction methods can be enhanced with experimental data.

Purpose of the Study:

  • To evaluate the optimal strategy for incorporating experimental data into membrane protein topology prediction.
  • To develop and apply a robust methodology for large-scale topology mapping of inner membrane proteins.

Main Methods:

  • Combining computational topology prediction with experimental determination of C-terminal location (cytoplasmic or periplasmic).
  • Assessing the efficacy of C-terminus versus internal loop experimental data for topology mapping.
  • Applying the validated methodology to 31 Escherichia coli inner membrane proteins.

Related Experiment Videos

Main Results:

  • Experimental determination of C-terminal location is superior to internal loop analysis for large-scale topology studies.
  • The developed methodology provides reliable topology models for inner membrane proteins.
  • Experimentally validated topology models were generated for 31 E. coli inner membrane proteins.

Conclusions:

  • The integration of C-terminal experimental data significantly improves membrane protein topology prediction accuracy.
  • This approach is scalable and suitable for genome-wide topology mapping initiatives.
  • The study provides valuable topological data for a set of E. coli inner membrane proteins, advancing structural and functional studies.