Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Amperometric ATP biosensor based on polymer entrapped enzymes.

Angelika Kueng1, Christine Kranz, Boris Mizaikoff

  • 1School of Chemistry and Biochemistry, Georgia Institute of Technology, Atlanta, GA 30332-0400, USA.

Biosensors & Bioelectronics
|March 30, 2004
PubMed
Summary

A novel dual enzyme electrode co-immobilizes glucose oxidase (GOD) and hexokinase (HEX) for sensitive adenosine-5'-triphosphate (ATP) detection. This biosensor offers improved performance at physiological pH, enabling new diagnostic tools.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Single-sequence based gFET-aptasensors for the discrimination of apo- and holo-RBP4 in human serum.

Scientific reports·2026
Same author

A Compact Multiplexed Hazardous Gas Sensing Platform for Real-Time Monitoring in Mining Scenarios.

ACS measurement science au·2026
Same author

Prussian Blue-based microsensors in short-circuit mode: a simplified approach for localized hydrogen peroxide detection.

Analytical and bioanalytical chemistry·2026
Same author

Multifactorial experimental design approach in forced degradation studies with a focus on the intensity of stress conditions - A trastuzumab case study.

International journal of biological macromolecules·2026
Same author

Scanning Photoelectrochemical Microscopy for the Investigation of Local Photocatalytic H<sub>2</sub> Evolution in Matrixed Langmuir Films.

ACS applied materials & interfaces·2026
Same author

Correction: Spectroscopic fingerprinting of extracellular vesicles from diverse cellular origins byATR-FTIR for vibrational biomarkers of vector-host interactions.

Scientific reports·2026

Area of Science:

  • Biosensors and electrochemical sensing
  • Enzyme immobilization techniques
  • Biochemical analysis

Background:

  • Adenosine-5 -triphosphate (ATP) is crucial for cellular energy and signaling.
  • Accurate ATP detection at physiological pH is vital for biological and medical research.
  • Existing ATP biosensors often face limitations in sensitivity, response time, or operational pH.

Purpose of the Study:

  • To develop a dual enzyme electrode for sensitive ATP detection.
  • To achieve ATP detection at physiologically relevant pH levels.
  • To improve the sensitivity, response time, and reproducibility of ATP biosensors.

Main Methods:

  • Co-immobilization of glucose oxidase (GOD) and hexokinase (HEX) using pH-shift induced polymer deposition.

Related Experiment Videos

  • Development of a simple electrochemical procedure for enzyme co-immobilization.
  • Fabrication of micro biosensors with controlled immobilization processes.

    • Main Results:

    • Achieved a detection limit of 10 nmol l(-1) for ATP.
    • Demonstrated significant improvements in sensitivity, response time, and reproducibility.
    • Successfully fabricated micro biosensors with a 25 micrometer diameter.
    • Validated ATP determination through enzyme competition for glucose.

    Conclusions:

    • The developed dual enzyme electrode offers a robust platform for ATP detection at physiological pH.
    • The pH-shift induced co-immobilization method enhances biosensor performance.
    • This technology provides a basis for next-generation, high-performance ATP biosensors.