Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

[Difficulties with immunofixation interpretation].

B Onraed1, M Nguyen, B Lerche

  • 1Laboratoire de biochimie, Hôpital cardiologique CHRU, Lille.

Annales De Biologie Clinique
|March 30, 2004
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Measurements of the <math></math> production cross section and the <math></math> branching fraction, and constraints on anomalous triple gauge couplings at <math> </math>.

The European physical journal. C, Particles and fields·2019
Same author

Combination of Searches for Higgs Boson Pair Production in Proton-Proton Collisions at sqrt[s]=13  TeV.

Physical review letters·2019
Same author

Measurement of associated production of a <math></math> boson and a charm quark in proton-proton collisions at <math> </math>.

The European physical journal. C, Particles and fields·2019
Same author

Measurement of b hadron lifetimes in pp collisions at <math> </math> <math></math>.

The European physical journal. C, Particles and fields·2019
Same author

Measurement of prompt and nonprompt charmonium suppression in <math></math> collisions at 5.02 <math></math>.

The European physical journal. C, Particles and fields·2019
Same author

Measurement of the <math></math> cross section in pp collisions at <math> </math> and validation of <math></math> lepton analysis techniques.

The European physical journal. C, Particles and fields·2019
Same journal

[The role of the heart-on-a-chip to drug evaluation].

Annales de biologie clinique·2026
Same journal

A morphology-driven proof-of-concept study linking interphase nuclear abnormalities to dicentric-mediated genomic instability.

Annales de biologie clinique·2026
Same journal

Analytical validation and clinical concordance of Diazyme free light chain assays on the Cobas® platform compared with Freelite™.

Annales de biologie clinique·2026
Same journal

Small-cell secondary plasma cell leukemia mimicking low-grade B-cell lymphoma.

Annales de biologie clinique·2026
Same journal

[Multiple myeloma and analytical discrepancy in serum free light chain assay: a case report].

Annales de biologie clinique·2026
Same journal

[Summary of the 34th National Conference of the National College of Hospital Biochemistry].

Annales de biologie clinique·2026
See all related articles

Immunofixation assays can present interpretation challenges, particularly with additives, variable antiserum avidity, and similar precipitin band migration. Regular reagent controls and using diverse antisera are crucial for accurate monoclonal gammopathy and Bence Jones proteinuria diagnosis.

Area of Science:

  • Clinical Chemistry
  • Immunology
  • Protein Electrophoresis

Background:

  • Immunofixation is a key diagnostic tool for monoclonal gammopathies and Bence Jones proteinuria.
  • Accurate interpretation is vital for patient management but can be challenging.

Observation:

  • Analysis of five cases highlights common interpretation difficulties in immunofixation.
  • Interferences from additives in seric immunofixation were observed.
  • Urinary immunofixation revealed Bence Jones proteinuria with variable complete monoclonal immunoglobulin presence.

Findings:

  • Variability in antiserum avidity can complicate results.
  • Similar migration patterns of precipitin bands pose interpretation challenges, as seen in a case of monoclonal IgA.
  • Reagent quality and consistency are critical for reliable immunofixation.

Related Experiment Videos

Implications:

  • Regular quality control of immunologic reagents is essential.
  • Employing antisera from different sources with the same specificity can aid in resolving complex cases.
  • Improved diagnostic accuracy in immunofixation can lead to better patient outcomes.