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Published on: July 1, 2016
Determination of warfarin at trace-levels in water by solid-phase spectrofluorimetry
J L Quero1, J Rohand, A N Montón
1Department of Analytical Chemistry, University of Granada, E-18071, Granada, Spain.
Abstract:
A new selective and sensitive spectrofluorimetric method for the determination of warfarin at trace levels (0.2-50.0 ng/ml) in water is proposed. Warfarin is fixed on Sephadex QAE A-25 gel (at pH = 7.0) and its fluorescence is measured directly in the solid-phase using a 1-mm silica cell at 312/385 nm with a detection/quantification limit of 0.06/0.2 ng/ml, a relative standard deviation of 2.3% and recoveries between 95 and 105%. The method is applied to the determination of warfarin residues in water.
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