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Related Experiment Videos

Utilization of nitrite as a nitrogen source by Botryococcus braunii.

Suling Yang1, Jun Wang, Wei Cong

  • 1State Key Laboratory of Biochemical Engineering, Institute of Process Engineering, CAS, P.O. Box 353, Beijing 100080, PR China.

Biotechnology Letters
|March 31, 2004
PubMed
Summary

Botryococcus braunii effectively uses nitrite as a nitrogen source, showing similar growth rates to nitrate. Higher nitrite concentrations supported substantial algal biomass accumulation.

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Area of Science:

  • * Biotechnology and Algal Physiology
  • * Microbial Nitrogen Metabolism

Background:

  • * Algae require nitrogen for growth, with nitrate being a common source.
  • * Investigating alternative nitrogen sources like nitrite is crucial for optimizing algal cultivation.

Purpose of the Study:

  • * To evaluate the efficacy of nitrite as a sole nitrogen source for Botryococcus braunii growth.
  • * To compare the growth performance and hydrocarbon production of B. braunii using nitrite versus nitrate.

Main Methods:

  • * Cultivation of Botryococcus braunii under different concentrations of nitrite (2 mM and 4 mM) and nitrate (4 mM).
  • * Monitoring of algal biomass doubling time, final biomass yield, and nitrogen source removal.
  • * Analysis of hydrocarbon composition in relation to nitrogen sources.

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Main Results:

  • * Nitrite at 2 mM did not impede growth and served as an effective nitrogen source, yielding a minimum biomass doubling time of 4.2 days.
  • * 4 mM nitrite supported rapid growth after a 10-day lag phase, reaching 1.1 g/l biomass, with 100% nitrite removal.
  • * Hydrocarbon production showed minimal differences across the tested nitrogen sources.

Conclusions:

  • * Nitrite is a viable and efficient nitrogen source for Botryococcus braunii cultivation.
  • * The choice of nitrogen source (nitrite vs. nitrate) has a limited impact on the hydrocarbon profiles of B. braunii.