Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Search research articles

Related Experiment Videos

Low-cost, frequency-domain, fluorescence lifetime confocal microscopy.

M J Booth¹, T Wilson

¹Department of Engineering Science, University of Oxford, Parks Road, Oxford OX1 3PJ, UK. martin.booth@eng.ox.ac.uk

Journal of Microscopy

|March 31, 2004

Summary

This study introduces a cost-effective frequency-domain fluorescence lifetime confocal microscope. It rapidly captures fluorescence intensity and lifetime images of biological samples and beads in real time.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Bed occupancy and nosocomial infections in the intensive care unit: A retrospective observational study in a tertiary hospital.

The Southern African journal of critical care : the official journal of the Critical Care Society·2024

Same author

Minimally invasive cerebral revascularization in moyamoya disease in adult patients.

Neuro-Chirurgie·2022

Same author

Post-operative volumes following endoscopic surgery for non-functioning pituitary macroadenomas are predictive of further intervention, but not endocrine outcomes.

BMC endocrine disorders·2021

Same author

Treatment Preferences for Cardiac Procedures of Patients With Chronic Kidney Disease in Acute Coronary Syndrome: Design and Pilot Testing of a Discrete Choice Experiment.

Canadian journal of kidney health and disease·2021

Same author

Addressing the pitfalls when designing intervention studies to discover and validate biomarkers of habitual dietary intake.

Metabolomics : Official journal of the Metabolomic Society·2019

Same author

Purified Human Pancreatic Islets, CIT Culture Media with Lisofylline or Exenatide.

CellR4-- repair, replacement, regeneration, & reprogramming·2019

Area of Science:

Biophysics
Optical Microscopy
Spectroscopy

Background:

Fluorescence lifetime imaging microscopy (FLIM) provides valuable functional and structural information.
Traditional FLIM systems can be complex and expensive.

Purpose of the Study:

To develop and implement an affordable frequency-domain fluorescence lifetime confocal microscope.
To demonstrate its capability for rapid imaging of biological specimens.

Main Methods:

Utilized switched diode laser illumination for frequency-domain measurements.
Employed standard radio-frequency electronics for modulation and phase-sensitive detection.
Integrated the system for real-time display of fluorescence intensity and lifetime images.

Related Experiment Videos

Main Results:

Successfully acquired fluorescence lifetime images of both bead phantoms and biological samples.
Demonstrated rapid image acquisition from a single confocal scan.
Validated the use of inexpensive electronics for advanced microscopy.

Conclusions:

The developed frequency-domain FLIM system offers a cost-effective and rapid alternative for biological imaging.
This approach enables real-time visualization of fluorescence lifetime, enhancing microscopic analysis.