Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Immunosensor with self-referencing based on surface plasmon diffraction.

Fang Yu1, Wolfgang Knoll

  • 1Max-Planck-Institute for Polymer Research, Ackermannweg 10, D-55128 Mainz, Germany.

Analytical Chemistry
|April 1, 2004
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

[Clinical application of Flow-through chimeric anterolateral thigh perforator flap].

Zhongguo xiu fu chong jian wai ke za zhi = Zhongguo xiufu chongjian waike zazhi = Chinese journal of reparative and reconstructive surgery·2018
Same author

Biological Processes and Biomarkers Related to Frailty in Older Adults: A State-of-the-Science Literature Review.

Biological research for nursing·2018
Same author

A new era for stroke therapy: Integrating neurovascular protection with optimal reperfusion.

Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism·2018
Same author

Two-Year Randomized Clinical Trial of Adjunctive Minocycline Microspheres in Periodontal Maintenance.

Journal of dental hygiene : JDH·2018
Same author

Effect of family education program on cognitive impairment, anxiety, and depression in persons who have had a stroke: A randomized, controlled study.

Nursing & health sciences·2018
Same author

Eimeria tenella infection perturbs the chicken gut microbiota from the onset of oocyst shedding.

Veterinary parasitology·2018
Same journal

Biodegradable Self-Powered Electrotherapy Patch for Integrated Smart Wound Management.

Analytical chemistry·2026
Same journal

Metabolite Fraction Libraries for Quantitative NMR Metabolomics.

Analytical chemistry·2026
Same journal

Self-Contained Lateral-Flow Microfluidic Bead-Based Assay for Rapid Quantification of Early-Stage Kidney Biomarkers.

Analytical chemistry·2026
Same journal

Overcoming the Debye Shielding Effect with Concave-Convex Structures for Sensitivity-Enhanced Thin-Film Transistors.

Analytical chemistry·2026
Same journal

Mode-Phase-Difference Photothermal Spectroscopy Assisted by a Bent Biconically Tapered Microfiber for Gas Sensing.

Analytical chemistry·2026
Same journal

Negative-Pressure-Actuated Microfluidics: A Dual-Mode Point-of-Care Sensor for Allergen-Specific IgE in Interstitial Fluid.

Analytical chemistry·2026
See all related articles

We developed a novel immunosensor using surface plasmon diffraction for real-time, label-free detection of binding events. This stable sensor achieved picomolar sensitivity for human chorionic gonadotropin (hCG) detection.

Area of Science:

  • * Plasmonics and Surface Science
  • * Biosensing and Immunoassay Development
  • * Nanomaterials and Nanotechnology

Background:

  • * Traditional immunosensors often require labels, adding complexity and cost.
  • * Real-time, in situ monitoring of binding events is crucial for understanding biological interactions.
  • * Bulk fluctuations can interfere with sensor signal stability and accuracy.

Purpose of the Study:

  • * To introduce a novel immunosensor utilizing surface plasmon diffraction.
  • * To demonstrate the sensor's capability for in situ, real-time, and label-free observation of interfacial binding.
  • * To evaluate the sensor's sensitivity and stability in detecting a target hormone.

Main Methods:

  • * Fabrication and characterization of a surface plasmon diffraction immunosensor.

Related Experiment Videos

  • * Implementation of a self-referencing mechanism for bulk fluctuation compensation.
  • * Direct detection of human chorionic gonadotropin (hCG) binding events.

    • Main Results:

    • * The surface plasmon diffraction immunosensor enabled label-free, real-time monitoring of binding.
    • * The inherent self-referencing mechanism effectively compensated for bulk fluctuations, as shown in temperature variation experiments.
    • * The sensor demonstrated a stable baseline signal and achieved picomolar sensitivity for hCG detection.

    Conclusions:

    • * A novel surface plasmon diffraction immunosensor offers a robust platform for biosensing.
    • * The sensor's self-referencing capability ensures signal stability and reliability.
    • * High sensitivity and label-free detection make this immunosensor suitable for various diagnostic applications, including hCG monitoring.