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Polykaryon formation using a swollen conidium of Trichoderma reesei
Hideo Toyama1, Makiko Yano, Takeshi Hotta
1Department of Health and Nutrition, Minamikyushu University, Kirishima 5-1-2, Miyazaki 880-0032, Japan. gaf00771@nifty.com
Applied Biochemistry and Biotechnology
|April 1, 2004
Summary
This study demonstrates a novel method for generating polykaryons in Trichoderma reesei using swollen conidia and colchicine. This technique enables the selection of cellulase hyperproducers without compromising growth rate.
Area of Science:
- Mycology
- Biotechnology
- Genetics
Background:
- Trichoderma fungi possess oval, mononucleate conidia.
- Conidia swell and become spherical in liquid media, increasing internal space.
- This swelling facilitates the formation of larger autopolyploid nuclei.
Purpose of the Study:
- To investigate polykaryon formation in swollen Trichoderma reesei conidia.
- To develop a method for selecting improved cellulase-producing strains.
- To explore autopolyploidization for enhanced fungal traits.
Main Methods:
- Swelling of Trichoderma reesei QM6a conidia in Mandel's medium.
- Autopolyploidization of swollen conidia using the mitotic arrester, colchicine.
- Induction of polykaryon formation from autopolyploidized swollen conidia.
Main Results:
- Autopolyploid nuclei were successfully generated in swollen conidia.
- Multiple smaller nuclei, similar in size to original nuclei, formed from autopolyploid nuclei.
- Polykaryon formation was achieved using swollen conidia.
Conclusions:
- Swollen conidia offer a viable platform for autopolyploidization and polykaryon formation.
- This method allows for the selection of cellulase hyperproducing Trichoderma strains.
- The approach maintains growth rate while enhancing cellulase production.