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Streptozotocin-induced alterations in rat liver Golgi complexes are ameliorated by BMOV
A M Kordowiak1, D Dziga, W Dabroś
1Department of General Biochemistry, Faculty of Biotechnology, Jagiellonian University, Krakow, Poland. anna@piryt.net
Abstract:
Twenty years ago, we detected the interdependence between structure and function of rat liver Golgi complexes that are characteristic for streptozotocin diabetes, which served us in further investigations as a useful indicator of the effectiveness of drugs we were testing. This work presented results obtained in eight groups of rats (four control and four diabetic) that were administered orally either bis(maltolato)oxovanadium(IV) [BMOV] or maltol alone. The activities of the rat liver Golgi marker enzyme, galactosyltransferase [GalT], as well as the morphology of Golgi complexes were studied in situ using an electron microscope; parallel estimations of vanadium concentration and phospholipid percentage were made in Golgi-rich preparations isolated from the liver. Our main findings were normalization in diabetic animals orally treated with 1.8 mmol BMOV in 0.09 mol NaCl solutions over seven days, which demonstrated an accompanying increase in phosphatidic acid (PA) percentage (p < 0.05) compared to controls. In the diabetic groups, Pearson's test showed a positive double correlation between GalT activity, vanadium concentration, and PA percentage in Golgi-rich membrane preparations from the liver. Additionally, a negative correlation was found between vanadium concentration and phosphatidylcholine percentage in the fractions.
Insights
Bis(maltolato)oxovanadium(IV) [BMOV] normalized diabetic rat liver Golgi complexes, increasing phosphatidic acid (PA) and galactosyltransferase (GalT) activity. This suggests BMOV
Area of Science:
- Biochemistry
- Cell Biology
- Pharmacology
Background:
- Streptozotocin-induced diabetes alters rat liver Golgi complex structure and function.
- Galactosyltransferase (GalT) activity in Golgi complexes serves as a marker for drug efficacy.
Purpose of the Study:
- To investigate the effect of bis(maltolato)oxovanadium(IV) [BMOV] on Golgi complex structure and function in diabetic rats.
- To assess the correlation between BMOV treatment, vanadium concentration, phospholipid changes, and GalT activity.
Main Methods:
- Oral administration of BMOV or maltol to control and diabetic rats.
- Electron microscopy to study Golgi complex morphology in situ.
- Isolation of Golgi-rich liver preparations for vanadium concentration and phospholipid analysis.
- Measurement of galactosyltransferase (GalT) activity.
Main Results:
- Oral BMOV treatment normalized Golgi complex morphology and GalT activity in diabetic rats.
- BMOV administration led to a significant increase in phosphatidic acid (PA) percentage.
- Positive correlation observed between GalT activity, vanadium concentration, and PA percentage.
- Negative correlation found between vanadium concentration and phosphatidylcholine percentage.
Conclusions:
- BMOV effectively reverses diabetes-induced alterations in rat liver Golgi complexes.
- Vanadium concentration in Golgi-rich fractions correlates with improved cellular function and altered phospholipid composition.
- BMOV shows therapeutic potential for managing diabetes-related cellular dysfunction.
