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Updated: Aug 25, 2026

Single-cell Analysis of Immunophenotype and Cytokine Production in Peripheral Whole Blood via Mass Cytometry
Published on: June 26, 2018
[Immunophenotype of peripheral blood mononuclear cells in atopic diseases]
Tomasz Pitsch1, Zenon Brzoza, Barbara Rogala
1Katedry i Kliniki Chorób Wewnetrznych, Alergologii i Immunologii Klinicznej w Zabrzu Slaskiej Akademii Medycznej w Katowicach. b-rogala pro.onet.pl
Abstract:
According to the different theories considering the role of CD30 antigen in the differentiation of T lymphocytes, the aim of this study was to analyze the immunophenotype of peripheral blood mononuclear cells (PBMC) in patients with mild atopic asthma and seasonal allergic rhinitis in out of season period. The immunophenotype of peripheral blood mononuclear cells (CD4, CD38, CD8, CD23, CD25, CD57, CD30, HLA-DR) was analyzed by flow-cytometry. A statistically significant rise in the percentage of CD30+ PBMCs in asthma group (3.50 +/- 2.07%) was seen when compared with allergic rhinitis group (1.80 +/- 0.90%). In allergic rhinitis patients a significant rise in the percentage of CD8+ lymphocytes (13.21 +/- 5.87%) was observed in comparison to asthma group (8.36 +/- 5.88%). No statistically significant differences in the expression of the other estimated molecules were detected. The enhancement of CD30 molecule expression on PBMCs in symptomatic asthma is supposed to confirm, due to Romagnani's theory, the predominance of Th2-type response in the pathogenesis of immunoinflammation active phase. PBMCs from patients with allergic rhinitis in out-of-season period may switch into cytotoxic/suppressive cells, confirmed by rise in the percentage of CD8+.
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