Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Genetically engineered fluorescent cell marker for labeling CD34+ hematopoietic stem cells.

Kaiming Ye1, Sha Jin, Jerome S Schultz

  • 1Center for Biotechnology and Bioengineering, Department of Bioengineering, University of Pittsburgh, 300 Technology Drive, Pittsburgh, Pennsylvania 15219, USA. kaiming@pitt.edu

Biotechnology Progress
|April 3, 2004
PubMed
Summary

Researchers engineered a CD34EGFP fluorescent marker for in vivo stem cell tracking. This marker utilizes the CD34 promoter to specifically label hematopoietic stem cells (HSC), enabling visualization and study.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Serum BP180 and BP230 autoantibodies in Alzheimer's disease: diagnostic and cognitive associations in a retrospective study.

Frontiers in aging neuroscience·2026
Same author

Connective tissue diseases combined with chronic myelomonocytic leukemia and myelofibrosis: a case report and literature review.

Frontiers in immunology·2026
Same author

Evaluation of the Effectiveness of Longitudinal Incision for Endoscopic Submucosal Excavation of Gastric Subepithelial Lesions.

Journal of visualized experiments : JoVE·2026
Same author

Mechanistic insights into cannabidiol-mediated TrkB activation via FRS2 interaction in attenuating Alzheimer's disease pathology and cognitive impairment.

Molecular psychiatry·2026
Same author

A novel cannabidiol-derived small molecule ameliorates lower urinary tract dysfunction in mice with spinal cord injury via suppressing neuroinflammation.

Life sciences·2026
Same author

Successful Treatment of Pityriasis Rubra Pilaris With Deucravacitinib.

JAMA dermatology·2026

Area of Science:

  • Molecular Biology
  • Stem Cell Research
  • Genetic Engineering

Background:

  • Labeling and tracking stem cells in vivo presents a significant challenge in biological research.
  • Existing methods may lack specificity or permanence for long-term studies.
  • Hematopoietic stem cells (HSCs) are crucial for blood formation and disease research.

Purpose of the Study:

  • To develop a novel fluorescent cell marker for specific in vivo labeling and tracking of CD34(+) stem cells.
  • To leverage the cell-specific activity of the CD34 promoter for targeted gene expression.
  • To establish a reliable method for visualizing and monitoring stem cell populations.

Main Methods:

  • Engineered a CD34EGFP fluorescent marker using a retroviral vector derived from a murine stem cell virus.

Related Experiment Videos

  • Utilized a 454 bp segment upstream of the murine CD34 cDNA as a cell-specific promoter.
  • Tested marker expression in various cell types, including CD34(+) HSCs, progenitor cells, CD34(-) cells, and terminally differentiated cells.

    • Main Results:

    • The CD34EGFP marker was successfully expressed in CD34(+) stem and progenitor cells.
    • No expression was observed in CD34(-) or terminally differentiated cells, confirming cell-specific labeling.
    • RT-PCR analysis showed comparable transcription levels between the CD34 promoter and CMV promoter in CD34(+) progenitor cells.

    Conclusions:

    • The engineered CD34EGFP marker provides a robust tool for specific labeling and in vivo tracking of CD34(+) hematopoietic stem cells.
    • This approach offers a versatile framework for creating various cell-specific fluorescent markers by combining different promoters and fluorescent proteins.
    • The findings facilitate advancements in stem cell research, regenerative medicine, and the study of hematological disorders.