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[DNA chip analysis of gene expression patterns in poorly-differentiated human stage I lung adenocarcinoma]
Guo-hong Xing1, Jin-chuan Zhang
1Respiratory Department of Nanlou, PLA General Hospital, Beijing 100853, China. xinggh90@sina.com
Objective:
To screen genes with abnormal expression in poorly-differentiated human lung adenocarcinoma at stage I with cDNA chip.
Methods:
The mRNA was extracted from cancer tissue and matched normal lung tissue, and was labeled by Cy5-dUTP or Cy3-dUTP as probes. Subsequently, the mixed probes were hybridized to the cDNA chip containing 8192 genes. The information was obtained by managing the cDNA chip with ScanArray4000 scanner and GenePix3.0 software.
Results:
Five hundred and eighty genes were differentially expressed between cancer and normal lung tissue. Compared with normal lung tissue, 405 genes were up-regulated and 175 genes were down-regulated in cancer tissue. These genes are involved in different cell activities such as growth regulation and signal transduction. Among the 66 genes with remarkable differential expression between the two tissues, 39 were up-regulated and 27 down-regulated.
Conclusion:
Many different kinds of genes are possibly involved in the initiation and progression of human lung adenocarcinoma. cDNA chip technique might be a useful method in screening lung cancer implicated genes.