Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Relationship between somatic mutation and neoplastic transformation.

J C Barrett, P O Ts'o

    Proceedings of the National Academy of Sciences of the United States of America
    |July 1, 1978
    PubMed
    Summary
    This summary is machine-generated.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    SACI-IO HR+: A randomized phase II trial of sacituzumab govitecan with or without pembrolizumab in patients with metastatic hormone receptor-positive/HER2-negative breast cancer.

    Annals of oncology : official journal of the European Society for Medical Oncology·2026
    Same author

    Agreement Across 10 Artificial Intelligence Models in Assessing Human Epidermal Growth Factor Receptor 2 (HER2) Expression in Breast Cancer Whole-Slide Images.

    Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc·2026
    Same author

    Enhanced patient selection with quantitative continuous scoring of PD-L1 expression for IO treatment in metastatic NSCLC.

    NPJ precision oncology·2025
    Same author

    Analysis of mutation status and homologous recombination deficiency in tumors of patients with germline BRCA1 or BRCA2 mutations and metastatic breast cancer: OlympiAD.

    Annals of oncology : official journal of the European Society for Medical Oncology·2021
    Same author

    PD-L1 and immune infiltrates are differentially expressed in distinct subgroups of gastric cancer.

    Oncoimmunology·2019
    Same author

    Rare-Variant Studies to Complement Genome-Wide Association Studies.

    Annual review of genomics and human genetics·2018
    Same journal

    Chemotactic self-organization captures the dynamics of mammalian hair follicle patterning.

    Proceedings of the National Academy of Sciences of the United States of America·2026
    Same journal

    Tomographic imaging of superconducting order using particle-hole interference.

    Proceedings of the National Academy of Sciences of the United States of America·2026
    Same journal

    Inhibitory potential of autologous neutralizing antibodies sets quantitative limits on the rebound-competent HIV-1 reservoir.

    Proceedings of the National Academy of Sciences of the United States of America·2026
    Same journal

    Inferring epidemiological parameters under an infectious phylogeography model with visitor dynamics.

    Proceedings of the National Academy of Sciences of the United States of America·2026
    Same journal

    Analytical modeling for suction cup designs for skin-interfaced wearable devices.

    Proceedings of the National Academy of Sciences of the United States of America·2026
    Same journal

    Improving cell-free metabolism through direct integration of artificial respiratory chains.

    Proceedings of the National Academy of Sciences of the United States of America·2026
    See all related articles

    Chemicals induce cell mutations and transformations, but neoplastic transformation is complex. This study shows it

    Area of Science:

    • Cellular and Molecular Biology
    • Toxicology
    • Carcinogenesis Research

    Background:

    • Somatic mutation and neoplastic transformation are key events in cancer development.
    • Understanding the relationship between mutation and transformation is crucial for carcinogen assessment.
    • Syrian hamster embryo cells are a model for studying these processes.

    Purpose of the Study:

    • To compare the kinetics and frequencies of somatic mutation and neoplastic transformation in hamster cells exposed to carcinogens.
    • To investigate whether somatic mutation assays can predict carcinogenic potential.
    • To explore the multistep nature of neoplastic transformation.

    Main Methods:

    • Induction of mutations in Syrian hamster embryo cells using benzo[a]pyrene and N-methyl-N'-nitro-N-nitrosoguanidine.

    Related Experiment Videos

  • Quantitation of mutations at hypoxanthine phosphoribosyltransferase and Na(+)/K(+) ATPase loci.
  • Assessment of phenotypic transformations including morphological changes and colony formation in agar.

    • Main Results:

    • Cellular transformations occurred at higher frequencies and with distinct kinetics compared to somatic mutations.
    • Morphological transformation was 25- to 540-fold more frequent than somatic mutation.
    • Colony formation in agar, a marker of neoplastic transformation, was detected much later than somatic mutations.

    Conclusions:

    • Neoplastic transformation is a complex, multistep process, not solely explained by single gene mutations.
    • Simple extrapolation from somatic mutation assays may not accurately predict carcinogenic potential.
    • Chemical carcinogens induce complex cellular changes beyond simple mutagenesis.