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Recent progress in biocatalyst discovery and optimization.

Dan E Robertson¹, Brian A Steer

¹Diversa Corporation, San Diego, CA 92121, USA. drobertson@diversa.com

Current Opinion in Chemical Biology

|April 6, 2004

Summary

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Enzymes offer natural advantages for industrial catalysis, driving demand for pure chemicals. Advanced discovery and optimization techniques enable enzyme use in demanding industrial settings.

Area of Science:

Biocatalysis and Industrial Chemistry

Background:

Enzymes are increasingly utilized in industrial catalysis due to their inherent benefits.
Growing demand for enantiomerically pure fine chemicals and a shift from traditional chemical processes favor enzyme-assisted methods.

Purpose of the Study:

To highlight the expanding role of enzymes in industrial catalysis.
To discuss the impact of new enzyme discovery and optimization technologies.

Main Methods:

Genomic approaches and laboratory evolution for enzyme discovery and optimization.
Development of intelligent, process-targeted assays for high-throughput screening.

Main Results:

Enzymes can now be applied under harsh industrial conditions.
Stringent selectivity is achievable with engineered enzymes.

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Conclusions:

Enzyme technology is crucial for sustainable and efficient industrial chemical production.
Advanced screening platforms are essential for leveraging enzyme potential in industry.