Oocyte numbers are reduced in developing mouse ovaries cultured in testis-conditioned medium

Reduced numbers of oocytes were present in fetal ovaries of 13 d post coitum (dpc) mice, cultured for 4 d in medium conditioned (-CM) for 2 d by 13 dpc testes, compared with ovaries maintained in standard unconditioned medium. This effect was abolished by heat-inactivation of the testis-CM. Electrophoretic analysis of conditioned media revealed differences between testis-CM and ovary-CM. Ovarian differentiation was otherwise unaffected by the testis-CM and gonadal volume was not significantly reduced. Organisation of ovigerous cords proceeded, even though the full complement of oocytes was absent, and connective tissue septa developed normally between the small cords. The reduction in oocyte numbers occurred without any inhibition of müllerian duct development. Since others, using transgenic mice, have shown, that higher concentrations of antimüllerian hormone are required to decrease oocyte numbers than are necessary for duct regression, our results suggest that an additional factor is involved in producing this modified effect.