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Related Experiment Videos

EGF receptor regulation in normal mouse mammary gland.

S Z Haslam1, L J Counterman, K A Nummy

  • 1Physiology Department, Michigan State University, East Lansing 48823-1101.

Journal of Cellular Physiology
|September 1, 1992
PubMed
Summary

Hormones like estrogen and progesterone can affect epidermal growth factor receptor (EGF-R) levels in mammary glands. This interaction depends on the developmental stage of the mammary gland in female mice.

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Area of Science:

  • Endocrinology
  • Developmental Biology
  • Cell Biology

Background:

  • Estrogen (E), progesterone (P), and epidermal growth factor (EGF) are key regulators of mammary gland growth and development.
  • The interaction between these factors and their receptors (ER, PR, EGF-R) in different cell types is not fully understood.
  • Understanding these interactions is crucial for comprehending hormone-dependent mammary growth regulation.

Purpose of the Study:

  • To investigate if estrogen and/or progesterone influence epidermal growth factor receptor (EGF-R) levels.
  • To determine which cell types (epithelial or stromal) are affected by these hormonal interactions.
  • To examine the impact of the mammary gland's developmental stage on these hormonal effects.

Main Methods:

  • Comparative analysis of ovariectomy and hormone treatments on EGF-R levels in immature (5-week-old) and mature (10-week-old) female mice.

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  • Characterization of EGF-R as a single class of high-affinity binding sites.
  • Assessment of EGF-R concentration in mammary epithelial and stromal cells.

    • Main Results:

    • EGF-R concentration was twofold higher in glands of 5-week-old mice compared to 10-week-old mice.
    • Ovariectomy did not significantly alter EGF-R concentration in either age group.
    • In 5-week-old mice, estrogen and/or progesterone had no effect on EGF-R levels in epithelial or stromal cells.
    • In 10-week-old mice, combined estrogen and progesterone treatment led to a twofold increase in EGF-R concentration within the mammary epithelium.

    Conclusions:

    • The developmental stage of the mammary gland significantly influences the interaction between EGF, estrogen, and progesterone.
    • Hormonal regulation of EGF-R levels is dependent on the age and maturity of the female mouse.
    • These findings highlight the complex interplay of hormones and growth factors in mammary gland development.