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Protein-protein interaction analysis by nuclear magnetic resonance spectroscopy
Guanghua Gao1, Jason G Williams, Sharon L Campbell
1Department of Biochemistry and Biophysics, University of North Carolina @ Chapel Hill, Chapel Hill, NC, USA.
Methods in Molecular Biology (Clifton, N.J.)
|April 6, 2004
Abstract:
Nuclear magnetic resonance (NMR) is a powerful technique to study protein-protein interactions in solution. Various methods have been developed and applied successfully for locating binding sites on proteins. The application of NMR chemical-shift perturbation to map the protein-protein interfaces is described in this chapter, providing a practical guideline that can be followed to carry out the experiments.