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Updated: Aug 25, 2026

FRET Microscopy for Real-time Monitoring of Signaling Events in Live Cells Using Unimolecular Biosensors
Published on: August 20, 2012
Microscopic analysis of fluorescence resonance energy transfer (FRET)
Brian Herman1, R Venkata Krishnan, Victoria E Centonze
1Department of Cellular and Structural Biology, University of Texas Health Science Center, San Antonio, TX, USA.
Abstract:
We describe here detailed practical procedures for implementing various approaches of fluorescence resonance energy transfer (FRET) in microscope-based measurements. A comprehensive theoretical formalism is developed and the different experimental procedures are outlined. A step-by-step protocol is provided for preparing the specimens for FRET measurements, data acquisition procedures, analysis, and quantification. Particular emphasis is given to exemplify the FRET applications in the study of protein-protein interactions.
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