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Related Experiment Video

Updated: Jul 23, 2026

Single Particle Cryo-Electron Microscopy: From Sample to Structure

Single Particle Cryo-Electron Microscopy: From Sample to Structure

Published on: May 29, 2021

TYSON: robust searching, sorting, and selecting of single particles in electron micrographs.

J R Plaisier¹, R I Koning, H K Koerten

¹Gorlaeus Laboratories, Leiden Institute of Chemistry, Leiden University, PO Box 9502, 2300 RA Leiden, The Netherlands. plaisier@chem.leidenuniv.nl

Journal of Structural Biology

|April 7, 2004

Summary

TYSON is a new program for fast, automatic, and semi-automatic particle selection in electron microscopy images. It efficiently identifies and sorts particles, improving cryo-electron microscopy workflows.

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Area of Science:

Structural Biology
Biophysics
Microscopy

Background:

Accurate particle selection is crucial for high-resolution cryo-electron microscopy (cryo-EM).
Manual particle selection is time-consuming and subjective.

Purpose of the Study:

To introduce TYSON, a novel software tool for automated and semi-automated particle selection in electron micrographs.
To provide an efficient and user-friendly solution for processing large cryo-EM datasets.

Main Methods:

TYSON utilizes a three-step strategy: searching, sorting, and selecting single particles.
Particle identification employs local averaging, template matching, or local variance methods.
Automated sorting based on probability of true positives and an interactive step for false positive removal.

Main Results:

TYSON demonstrated efficiency in selecting particles from both spherical virus and asymmetric particle cryo-EM datasets.
The software enables rapid particle selection, with up to 20,000 particles processed in a single day.
The interactive step allows for quick elimination of false positives.

Conclusions:

TYSON offers a fast and effective solution for particle selection in cryo-EM.
The program streamlines the initial stages of cryo-EM data processing, facilitating higher throughput.
TYSON is suitable for both spherical and asymmetric particle analysis.