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Enzyme immunoassay and immunochemical characterization of pancreatic stone protein in human serum
T Katsuzaki1, N Tatemichi, C Takeichi
1Department of Clinical Chemistry, Maruko Pharmaceutical Co., Ltd., Kasugai, Japan.
Journal of Clinical Laboratory Analysis
|January 1, 1992
Abstract:
Monoclonal antibodies were raised against human pancreatic stone protein (PSP) and used for one-step enzyme immunoassay (EIA). PSP-S2-5 was employed as the standard in the assay. The assay's measurable range was 25-1,500 ng/ml and within run coefficient of variation was 3.7-6.4%. Analytical recovery of the assay was 101.5 +/- 5.65% (mean +/- SD). The results of experiments in which serum was fractionated by Mono S (cation exchange chromatography) suggested that most of immunoreactive material in human serum is PSP-S2-5. The EIA offers simple, rapid, and specific analysis of serum PSP level for clinical diagnosis.