On the use of post-transcriptional processing elements in transgenes
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Updated: Aug 14, 2026

Transient Gene Expression in Tobacco using Gibson Assembly and the Gene Gun
Published on: April 19, 2014
C B A Whitelaw1, L Hiripi, E Farini
1Department of Gene Expression and Development, Roslin Institute (Edinburgh), Roslin, Midlothian, EH25 9PS, UK. bruce.whitelaw@bbsrc.ac.uk
Abstract:
RNA processing events modulate final productivity of a given transgene. We have evaluated a series of RNA elements for their ability to enhance alpha1-antitrypsin production in mammary cells. Our results indicate the need for a case-by-case assessment of each construct design and the occurrence of gene silencing events in vivo.
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