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Anti-virulent Disruption of Pathogenic Biofilms using Engineered Quorum-quenching Lactonases
Published on: January 1, 2016
[Amplification of Lactobacillus bulgaricus beta-galactosidase gene and optimization of the reaction condition]
Chuan Wang1, Chao-wu Zhang, Xiao-fang Pei
1Department of Medical Technology, West China School of Public Health, Sichuan University, Chengdu 610041, China.
Objective:
To obtain the gene which encode high activity beta-galactosidase from Lactobacillus bulgaricus and study the influencing factors of amplification.
Methods:
Lysozyme and freeze-thaw cycles were applied to obtain Lactobacillus bulgaricus DNA; different template concentration and annealing temperature were selected for the amplification.
Results:
60 ng/microliter template concentration and 66 degrees C annealing temperature were the best reaction conditions of amplifying the gene.
Conclusion:
This study optimized the template concentration and anneal temperature conditions of amplifying Lactobacillus bulgaricus beta-galactosidase gene, thus providing a foundation for further studies.
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