Identifying Two Common Types of Breast Benign Diseases Based on Multiphoton Microscopy

Area of Science:

• Biomedical Optics
• Medical Imaging
• Pathology

Background:

• Multiphoton microscopy (MPM) is gaining traction for breast cancer research using two-photon excited fluorescence (TPEF) and second-harmonic generation (SHG).
• Breast benign diseases are significantly more common than breast cancer, affecting up to 30% of women and often requiring medical intervention.

Purpose of the Study:

• To investigate the application of MPM for imaging common breast benign diseases, specifically fibroadenoma and fibrocystic lesions.
• To assess MPM's capability in differentiating microstructural characteristics of normal breast tissue and benign pathological conditions.

Main Methods:

• Multiphoton microscopy (MPM) was employed to visualize the microstructural details of breast tissue samples.
• The study focused on analyzing TPEF and SHG signals to characterize tissue morphology.

Main Results:

• MPM successfully identified the microstructure of lobules and stroma in normal breast tissue.
• The technique revealed the interaction between compressed ducts and collagen fiber in fibroadenoma.
• MPM visualized the distinct architecture of cysts filled with fluid in fibrocystic disease.

Conclusions:

• MPM demonstrates the capability to differentiate microstructural features of normal breast tissue and common benign diseases.
• Integrating MPM into clinical imaging systems could enable real-time in vivo diagnosis of breast benign diseases.
• MPM holds potential for the simultaneous diagnosis of both benign breast conditions and breast cancer.