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Fluorometric assay protocol for protease-catalyzed transesterification reactions in organic solvents
Min Su Han1, Sang Oh Jung, Mahn-Joo Kim
1Center for Integrated Molecular Systems, and National Research Laboratory of Chirotechnology, Department of Chemistry, Pohang University of Science and Technology, San 31 Hyojadong, Pohang 790-784, Korea.
The Journal of Organic Chemistry
|April 13, 2004
Abstract:
A flourometric assay protocol for a subtilisin-catalyzed transesterification reaction in n-hexane has been developed. The method makes use of a Michael acceptor that forms a fluorescent adduct with thiophenol, one of the products generated in the transesterification reaction. The method may be employed for screening a biocatalyst useful for transesterification reactions in organic solvents and for optimizing the transesterification reaction conditions.