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Updated: Aug 24, 2026

Isolation, Characterization, And High Throughput Extracellular Flux Analysis of Mouse Primary Renal Tubular Epithelial Cells
Published on: June 20, 2018
Metabolism of tetraglycine in isolated non-filtering rat kidneys
Helena Alves de Carvalho Sampaio1, Krishnamurti de Morais Carvalho, Manassés Claudino Fonteles
1Curso de Doutorado em Farmacologia/Universidade Federal do Ceará, Fortaleza-CE, Brazil. reitor@uece.br
Abstract:
The purpose of this work was to study the metabolism of tetraglycine in the isolated non filtering rat kidney. Kidneys were perfused with Krebs-Henseleit solution containing 1 mM of tetraglycine. The determination of the peptidic residues and their components was done in an aminoacid microanalyzer. The results showed that tetraglycine was significantly oxidized to a maximal concentration of 0.12 mM at the end of 120 minutes (88%). The peptide was degraded to its constituents, diglycine and glycine. There was no elution of triglycine. The tetrapeptide produced glycine in increasing concentrations while diglycine as a metabolite was almost constant up to 80 minutes of perfusion. The hydrolysis of tetraglycine allowed us to believe that aminopeptidase, carboxypeptidase and dipeptidyl dipeptidase can be present in the contraluminal membranes of the rat kidney contributing to the handling of tetraglycine. Our results indicate newer pathways of the tetrapeptide metabolism.
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