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ent-Kaurenoic acids from Mikania hirsutissima (Compositae)
Emika Ohkoshi1, Satoshi Kamo, Mitsuko Makino
1College of Pharmacy, Nihon University, 7-7-1 Narashinodai, Funabashi, Chiba 274-8555, Japan.
Phytochemistry
|April 15, 2004
Summary
Two ent-kaurenoic acid derivatives from Mikania hirsutissima demonstrated significant lymphocyte proliferative activity. These compounds, 1 and 2, show potential for immune system modulation in peripheral blood mononuclear cells (hPBMC).
Area of Science:
- Phytochemistry
- Immunology
- Natural Products Chemistry
Background:
- Mikania hirsutissima (Compositae) is a plant source of bioactive compounds.
- Ent-kaurenoic acid derivatives are known for diverse biological activities.
Purpose of the Study:
- To isolate and identify compounds from Mikania hirsutissima aerial parts.
- To evaluate the proliferative activity of isolated compounds on human peripheral blood mononuclear cells (hPBMC).
Main Methods:
- Extraction of aerial parts of Mikania hirsutissima using 50% aqueous acetone.
- Isolation and purification of compounds using chromatographic techniques.
- Assessment of lymphocyte proliferation in hPBMC using specific assays.
Main Results:
- Four new ent-kaurenoic acid derivatives (1-4) and five known compounds (5-9) were isolated.
- Compounds 1 and 2 exhibited significant lymphocyte proliferative activity at 100 microM (43.8% and 36.7%, respectively).
Conclusions:
- Compounds 1 and 2, identified as 2beta,16alpha,17-trihydroxy-ent-kauran-19-oic acid and 3beta,16alpha,17-trihydroxy-ent-kauran-19-oic acid, possess notable immunomodulatory potential.
- These findings suggest Mikania hirsutissima as a source of novel compounds with lymphocyte-stimulating properties.