Related Experiment Video
Updated: Aug 24, 2026

Identification of Potential Anti-TB Candidates: A Step-by-Step Guide to Synthesis, MIC Determination, and Cytotoxicity Assessment in Mammalian Cells
Published on: May 22, 2026
Synthesis and antimycobacterial activity of 1,2,4-triazole 3-benzylsulfanyl derivatives
Vera Klimesová1, Lenka Zahajská, Karel Waisser
1Department of Inorganic and Organic Chemistry, Faculty of Pharmacy, Charles University, 50005 Hradec Králové, Czech Republic. klimeso@faf.cuni.cz
Abstract:
Series of 3-benzylsulfanyl derivatives of 1,2,4-triazole and 4-methyl-1,2,4-triazole were synthesized by alkylation of starting triazole-3-thiol with appropriately substituted benzyl halide. All members of the set were evaluated for in vitro antimycobacterial activity against Mycobacterium tuberculosis, M. avium, and two strains of M. kansasii. The activities were expressed as the minimum inhibitory concentration. The compounds exhibited only a moderate or slight antimycobacterial activity. Minimum inhibitory concentrations fall into a range of 32->1000 micromol/l. The most active substances bear two nitro groups or a thioamide group on the benzyl moiety. As regards the cytotoxicity effect, the evaluated compounds can be considered as moderately toxic.
Related Concept Videos
Antifungal Agents
Antiprotozoal Agents
Preparation and Reactions of Sulfides
Aryldiazonium Salts to Azo Dyes: Diazo Coupling
Anthelminthic Agents
