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Related Experiment Videos

A benchmark study with sealchip planar patch-clamp technology.

Jia Xu1, António Guia, David Rothwarf

  • 1AVIVA Biosciences Corp, San Diego, CA 92121, USA. jxu@avivabio.com

Assay and Drug Development Technologies
|April 20, 2004
PubMed
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This study benchmarks biochip technology for high-throughput electrophysiology, achieving over 75% success in gigaohm seals for rapid ion channel screening.

Area of Science:

  • Biophysics
  • Electrophysiology
  • Microfluidics

Background:

  • Conventional patch-clamp electrophysiology is information-rich but limited by low throughput and high costs.
  • Microchip-based systems offer miniaturization, faster perfusion, and reduced reagent costs for high-throughput screening.
  • Developing robust biochip technology is crucial for advancing drug discovery and ion channel research.

Purpose of the Study:

  • To establish a performance benchmark for a novel microchip-based patch-clamp system.
  • To validate the microfabrication and processing consistency of the biochips.
  • To assess the system's suitability for high-throughput compound screening.

Main Methods:

  • Sequential testing of 52 biochips to evaluate microfabrication consistency.

Related Experiment Videos

  • Performing planar whole-cell patch clamping experiments.
  • Measuring key electrophysiological parameters including seal resistance, access resistance (Ra), and membrane resistance (Rm).

    • Main Results:

    • Consistent hole size achieved through mature microfabrication and processing.
    • Demonstrated high-quality planar whole-cell patch clamping with >75% success rate for gigaohm seals.
    • Achieved stable whole-cell access (>15 min) with low access resistance (<15 MΩ) and high membrane resistance (>200 MΩ).

    Conclusions:

    • The developed biochip technology is mature and reliable for electrophysiological studies.
    • The system demonstrates high success rates and stable recordings, suitable for demanding applications.
    • These biochips are well-suited for high-throughput compound screening targeting ion channels.