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Related Experiment Videos

High-speed label-free detection by spinning-disk micro-interferometry.

Manoj M Varma1, Halina D Inerowicz, Fred E Regnier

  • 1Adaptive Optics and Biophotonics Laboratory, Department of Physics, Purdue University, West Lafayette, IN 47907-2036, USA.

Biosensors & Bioelectronics
|April 20, 2004
PubMed
Summary
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New spinning-disk interferometers offer sensitive, high-speed detection of biomolecules. This label-free technology, the BioCD, can screen thousands of proteins per disk with high precision.

Area of Science:

  • Analytical Chemistry
  • Biophysics
  • Materials Science

Background:

  • Spinning-disk interferometers represent a novel class of analytical sensors.
  • These devices utilize surface-normal self-referencing interferometers for biomolecule detection.
  • The technology is analogous to an optical CD but operates on microdiffraction quadrature principles.

Purpose of the Study:

  • To introduce and demonstrate the principles of the BioCD, a spinning-disk interferometer system.
  • To showcase the potential for high-speed and high-sensitivity detection of immobilized biomolecules.
  • To validate the fabrication and performance of interferometric microstructures on various disk materials.

Main Methods:

  • Fabrication of micro-interferometric structures on silicon and dielectric mirror disks.

Related Experiment Videos

  • Utilizing microdiffraction quadrature for sensitive, linear detection of bound molecules.
  • Testing the system with immobilized anti-mouse IgG and varying concentrations of mouse IgG.

    • Main Results:

    • Successful detection of immobilized anti-mouse IgG.
    • Quantification of specific mouse IgG binding at 10 femtomol levels.
    • Achieved a high sampling rate of 100 kilo-samples/s with negligible non-specific binding.
    • Demonstrated the potential for integrating over a million interferometric elements per disk.

    Conclusions:

    • Spinning-disk interferometers (BioCD) provide a label-free, high-speed, and sensitive method for biomolecule detection.
    • The small footprint of individual interferometers allows for massive parallelization.
    • This technology holds significant potential for high-throughput screening of proteins and other biomolecules.