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Updated: Aug 24, 2026
Use of In Vivo Single-fiber Recording and Intact Dorsal Root Ganglion with Attached Sciatic Nerve to Examine the Mechanism of Conduction Failure
Published on: August 27, 2019
Regional differences in blood-nerve barrier function and tight-junction protein expression within the rat dorsal root
Hisanori Hirakawa1, Seiichiro Okajima, Takanori Nagaoka
1Department of Pathology and Cell Regulation, Graduate School of Medical Science, Kyoto Prefectural University of Medicine, Kawaramachi Hirokoji, Kamigyo-ku, Kyoto, 602-8566, Japan.
Abstract:
To elucidate blood-nerve barrier function and tight-junction protein expression in the dorsal root ganglion (DRG), we analyzed the vascular permeability in the rat DRG by i.v. administration of fluorescent Evans-blue albumin (EBA) and compared it with the localization of claudin-1, claudin-5, and occludin by immunoconfocal microscopy. In the cell body-rich area within the DRG, extravascular leakage of EBA was noted and claudin-5 but neither claudin-1 nor occludin was detected. Conversely, in the nerve fiber-rich area within the DRG, no extravascular leakage of EBA was observed and both claudin-5 and occludin but no claudin-1 were detected in the blood vessel. These results demonstrate regional differences in the blood-nerve barrier function and tight-junction protein expression within the DRG.
