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Raman Spectroscopy Instrumentation: Overview

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A conventional Raman spectrophotometer includes a laser source, a sample holding system, a wavelength selector, and a detector.
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Related Experiment Video

Updated: Jun 19, 2026

Highly-Multiplexed Tissue Imaging with Raman Dyes

Highly-Multiplexed Tissue Imaging with Raman Dyes

Published on: April 21, 2022

Spatially multiplexed random-access Raman probe for in vivo tissue measurements.

Wataru Sakata¹, Atsuko Fujihara², Taisuke Ota³

¹Department of Applied Physics, Graduate School of Engineering, The University of Osaka, Osaka, Japan.

Biomedical Optics Express

|June 18, 2026

Summary

This summary is machine-generated.

We developed a novel Raman spectroscopy probe for rapid, label-free tissue identification in vivo. This high-throughput system achieves accurate nerve discrimination, paving the way for enhanced surgical navigation.

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An Intra-Tissue Radiometry Microprobe for Measuring Radiance In Situ in Living Tissue

An Intra-Tissue Radiometry Microprobe for Measuring Radiance In Situ in Living Tissue

Published on: June 2, 2023

Area of Science:

Biomedical Optics
Spectroscopy
Surgical Technology

Background:

Raman spectroscopy offers label-free biological tissue identification.
Clinical use is hindered by low-throughput spectral acquisition.
Need for faster, in vivo methods for intraoperative guidance.

Purpose of the Study:

To develop a high-throughput, spatially multiplexed Raman probe for simultaneous in vivo spectral acquisition.
To enable rapid, label-free intraoperative tissue identification.
To improve surgical navigation accuracy.

Main Methods:

Developed a spatially multiplexed random-access Raman probe.
Utilized a custom fiber bundle and spatial light modulator for parallel spectral acquisition (up to 1718 spectra per exposure).
Validated in vivo measurements on canine peripheral nerves and discriminant analysis on rat nerve/non-nerve tissues.

Main Results:

Demonstrated feasibility for intraoperative tissue identification in vivo within 5 seconds.
Achieved 92.5% accuracy and sensitivity for nerve discrimination using discriminant analysis.
Enabled simultaneous spectral acquisition from multiple, arbitrarily selected locations.

Conclusions:

The developed Raman probe significantly increases spectral acquisition throughput for in vivo measurements.
The system facilitates efficient and accurate intraoperative tissue identification, particularly for nerve discrimination.
This technology provides a foundation for advanced surgical navigation systems.