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The anti-delta suppressed mouse.

J E Layton, G R Johnson, D W Scott

    European Journal of Immunology
    |May 1, 1978
    PubMed
    Summary
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    Early B cell development involves immunoglobulin D (IgD). Suppressing IgD in mice reduced IgG antibody responses, impacting B cell function and antibody production.

    Area of Science:

    • Immunology
    • Developmental Biology
    • B cell biology

    Background:

    • Immunoglobulin D (IgD) is a crucial B cell surface receptor.
    • The developmental timeline and functional significance of IgD in early life remain incompletely understood.

    Purpose of the Study:

    • To investigate the ontogeny and functional role of IgD in neonatal and adult mice.
    • To develop a method for suppressing IgD-positive B cells to study their impact on immune responses.

    Main Methods:

    • Immunofluorescence staining to track IgD-positive cells in spleen and lymph nodes.
    • Development of an experimental system using anti-delta allotype serum to suppress IgD expression from birth.
    • In vitro and in vivo assessment of immune responses (IgM and IgG) in suppressed and control mice.

    Related Experiment Videos

    Main Results:

    • IgD-positive B cells first appeared in the spleen around 3 days of age and in lymph nodes by 4-5 days.
    • Suppression of IgD-positive cells led to a significant reduction in mu-positive (IgM) B cells (approx. 50%) without cell death, indicating modulation.
    • Suppressed mice showed a normal IgM response but a marked reduction in the IgG response.

    Conclusions:

    • IgD expression is a critical developmental event in B cells.
    • Suppression of IgD leads to altered B cell populations and significantly impairs IgG antibody production.
    • These findings highlight IgD's role in regulating B cell maturation and adaptive immunity, particularly IgG responses.