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Updated: Aug 24, 2026
High-throughput Physical Mapping of Chromosomes using Automated in situ Hybridization
Published on: June 28, 2012
The physical gene Hsp70 map on polytene chromosomes of Anopheles darlingi from the Brazilian Amazon
Míriam Silva Rafael1, Wanderli Pedro Tadei, Fiona F Hunter
1Coordenação de Pesquisas em Ciências da Saúde, Instituto Nacional de Pesquisas da Amazônia, Av. André Araújo, 2936, Bairro Petrópolis, 69083-000, Manaus, AM, Brasil. msrafael@inpa.gov.br
Abstract:
In situ hybridization was used to determine the physical location of the Hsp70 genes in salivary polytene chromosomes of Anopheles darlingi from Manaus and Macapá, Brazil, and to assess the usefulness of the Hsp70 locus as a genetic marker in A. darlingi populations. In both populations, the double markings corresponding to the Hsp70-12A and Hsp70-14A genes were located on the right arm of chromosome 2. The Hsp70 locus was considered to be an excellent marker for studying chromosomal evolution and relationships among A. darlingi populations.
