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ReAsH/FlAsH Labeling and Image Analysis of Tetracysteine Sensor Proteins in Cells
Published on: August 31, 2011
A supramolecular approach to protein labeling. A novel fluorescent bioassay for concanavalin a activity
Oleksandr Rusin1, Vladimír Král, Jorge O Escobedo
1Department of Chemistry, Louisiana State University, Baton Rouge, Louisiana 70803, USA.
Abstract:
[reaction: see text] A new method for the bioassay of concanavalin A is based on the interaction of saccharide-substituted oligopyrrolic macrocycles with lectins. A general sensing mechanism involves the interaction of aggregated and primarily nonfluorescent labels with the target protein, label deaggregation, site-specific binding, and fluorescence signaling. Addition of saccharides to the fluorescent lectin-macrocycle complex leads to the release of the label and partial quenching of fluorescence due to reaggregation. Specificity of the protein-probe binding is discussed.
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